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px330a puro 1x2 vector  (Addgene inc)


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    Addgene inc px330a puro 1x2 vector
    Px330a Puro 1x2 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/px330a+plasmid/pX330A-1x2+(Plasmid+%2358766)/pm41780532-799-13-15
    Average 94 stars, based on 83 article reviews
    px330a puro 1x2 vector - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Clone Assay:

    Article Title: APEX2‐mediated RAB proximity labeling identifies a role for RAB21 in clathrin‐independent cargo sorting
    Article Snippet: .. Briefly, three independent gRNAs cloned into the PX330A plasmid were cotransfected with the pEGFP‐Puro plasmid (addgene #45561) at a ratio of 1:1:1:0.25. ..

    Plasmid Preparation:

    Article Title: APEX2‐mediated RAB proximity labeling identifies a role for RAB21 in clathrin‐independent cargo sorting
    Article Snippet: .. Briefly, three independent gRNAs cloned into the PX330A plasmid were cotransfected with the pEGFP‐Puro plasmid (addgene #45561) at a ratio of 1:1:1:0.25. ..



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    Image Search Results


    The function exploration of genetic variants around GYS1 . Expression quantitative trait loci (eQTL) box plot. Box plot displaying the effect of the genotypes on gene expression levels in kidney tissues. Genotypic effects of ( A ) SNPs rs111518556 and ( B ) rs116573491 on expression of GYS1. The y axis shows the gene expression levels, and the x axis shows the genotypes of SNPs. The box plot displays the first and third quartiles (top and bottom of the boxes), the median (band inside the boxes), and the lowest and highest point within 1.5 times the interquartile range of the lower and higher quartiles. ( C ) The functional annotation for SNPs located near GYS1 in ENCODE and Roadmap. CRIPSR-dCas9-VP64-mediated transcriptional activation ( D ) in HEK293T cells on rs11518556 ( E ) and rs116573491 ( F )

    Journal: Genome Medicine

    Article Title: Transcriptome-wide association study revealed novel causal genes of renal-biopsy proven diabetic nephropathy

    doi: 10.1186/s13073-025-01590-x

    Figure Lengend Snippet: The function exploration of genetic variants around GYS1 . Expression quantitative trait loci (eQTL) box plot. Box plot displaying the effect of the genotypes on gene expression levels in kidney tissues. Genotypic effects of ( A ) SNPs rs111518556 and ( B ) rs116573491 on expression of GYS1. The y axis shows the gene expression levels, and the x axis shows the genotypes of SNPs. The box plot displays the first and third quartiles (top and bottom of the boxes), the median (band inside the boxes), and the lowest and highest point within 1.5 times the interquartile range of the lower and higher quartiles. ( C ) The functional annotation for SNPs located near GYS1 in ENCODE and Roadmap. CRIPSR-dCas9-VP64-mediated transcriptional activation ( D ) in HEK293T cells on rs11518556 ( E ) and rs116573491 ( F )

    Article Snippet: Human embryonic kidney (HEK293T) cells were purchased from the American Type Culture Collection (ATCC), HEK293T was grown in Dulbecco’s modified Eagle’s Medium (DMEM) (VivaCell, C3114-0500) supplemented with 10% FBS + 1% penicillin/streptomycin. pX330a dCas9-VP64 was a gift from Tatjana Sauka-Spengler (Addgene plasmid # 92,363), sgRNAs guide sequences were cloned into pSpCas9 (BB)−2A-Neo which was a gift from Ken-Ichi Takemaru (Addgene plasmid # 127,762).

    Techniques: Expressing, Gene Expression, Functional Assay, Activation Assay